New Granulocyte Antigens Demonstrated by Microgranulocytotoxicity Assay

New granulocyte antigens demonstrated by microgranulocytotoxicity assay.

Although granulocyte transfusions and bone marrow transplantation are becoming common clinical modalities, our knowledge of surface nonerythroid, nonlymphoid, non-HLA hematopoetic antigens remains very incomplete. Accordingly, we have systematically screened sera from recipients of multiple granulocyte and whole blood transfusions, and immunoneutropenic patients for antibodies directed primaril...

Adult Brain Antigens Demonstrated in Chick Embryos by Fractionated Antisera.

T H E appearance, during development, of antigens which react with antisera against specific adult antigens has been considered to reflect the synthesis of similar substances in the embryo (Clayton, 1960). Within the recognized limitations of serological specificities, where the adult antigens are characteristic of a particular organ or tissue, the appearance in the embryo of cross-reacting sub...

Antigens by Enzyme-Linked Immunosorbent Assay

A new type of immunoassay (enzyme-linked immunosorbent assay, ELISA) has been used for the detection of antibodies against Salmonella 0 antigens. The results show that ELISA is specific and highly sensitive. When compared with the Widal reaction, passive hemagglutination, and quantitative precipitation, ELISA was shown to be more sensitive. In addition, immunoglobulin G and immunoglobulin M ant...

New Possibilities Demonstrated by Micro-satellites

Anti-granulocyte antibody screening with extracted granulocyte antigens by a micro-mixed passive hemagglutination method.

BACKGROUND AND OBJECTIVES Serologic tests for granulocyte antibodies, i.e., the granulocyte agglutination test and the granulocyte immunofluorescence test, require panels of typed granulocytes that cannot be preserved for more than a few hours. We have developed a new method in which granulocyte antigens, extracted into saline containing 3% sucrose, are coated onto U-type Terasaki plates. With ...